Expression of cyclooxygenase-2 and relationship with mismatch repair gene and microsatellite instability in hereditary non-polyposis colorectal cancer

• Published in: Zhong hua yi xue za zhi Vol. 89; no. 20; p. 1377
• Main Authors: Zhang, Ying-hui, Sheng, Jian-qiu, Geng, Hong-gang, Han, Min, Huang, Ji-sheng, Mu, Hong, Han, Wen-liang, Chen, Ji-gui, Niu, Hong-li, Li, Ai-qin, Wu, Zi-tao, Li, Shi-rong
• Format: Journal Article
• Language: Chinese
• Published: China 26.05.2009
• Subjects: Adaptor Proteins, Signal Transducing - metabolism; Adult; Aged; Aged, 80 and over; Colorectal Neoplasms, Hereditary Nonpolyposis - genetics; Colorectal Neoplasms, Hereditary Nonpolyposis - metabolism; Cyclooxygenase 2 - metabolism; DNA Mismatch Repair; DNA-Binding Proteins - metabolism; Female; Gene Expression Regulation, Neoplastic; Humans; Microsatellite Instability; Middle Aged; MutL Protein Homolog 1; MutS Homolog 2 Protein - metabolism; Nuclear Proteins - metabolism; Young Adult
• ISSN: 0376-2491

To investigate the expression of Cyclooxygenase (COX)-2 and the relationship between cox-2, mismatch repair gene (MMR) proteins and microsatellite instability (MSI) in HNPCC. Twenty-eight cases of adenomas and 14 cases of carcinomas were collected from 33 HNPCC families patients by colonoscopy. Sporadic adenomas (n = 32) and carcinomas (n = 24) were used as a control group. The expressions of COX-2 and mismatch repair gene hMLH1, hMSH2, hMSH6 proteins were examined by immunohistochemistry. MS1 were analyzed by using PCR with BAT25, BAT26, D2S123, D5S346 and D17S250 loci. The COX-2 high-expression rates were 53.6% (15/28) and 42.9% (6/14) in HNPCC adenomas and carcinomas, and were 62.5% (20/32) and 91.7% (22/24) in sporadic adenomas and carcinomas. COX-2 expression was lower in HNPCC carcinomas than that of sporadic carcinomas (P < 0.05). MMR deficiency rate and positive rate of MSI-H were both 71.4% (10/14) respectively in HNPCC carcinomas. It was higher than that in sporadic colorectal carcinomas [both 12.5%