Induction of protective antibody response in rabbits against fascioliasis with Toxocara/Fasciola cross- reactive defined antigen

• Published in: Journal of the Egyptian Society of Parasitology Vol. 34; no. 3 Suppl; p. 1105
• Main Authors: Abdel-Megeed, Kadria N, Abdel-Rahman, Eman H
• Format: Journal Article
• Language: English
• Published: Egypt 01.12.2004
• Subjects: Animals; Antibodies, Helminth - biosynthesis; Antigens, Helminth - immunology; Chromatography, Affinity; Cross Reactions; Electrophoresis, Polyacrylamide Gel; Enzyme-Linked Immunosorbent Assay; Fasciola hepatica - immunology; Fascioliasis - prevention & control; Rabbits - immunology; Rabbits - parasitology; Toxocara - immunology; Vaccination
• ISSN: 1110-0583

A method of affinity chromatography purification of Toxocara vitulorum antigen cross- reacts with Fasciola gigantica antiserum is described. Characterization of the isolated cross- reactive fraction by SDS-polyacrylamide gel electrophoresis, isoelectric focusing and amino acid analysis resulted in a fraction consists of five polypeptides of 137.7KDa, 81KDa, 75KDa, 48KDa and 21.6KDa with isoelectric points of 8, 7.5, 7.2, 6.7 and 6.6. Seventeen amino acids were identified in the fraction with high proportions of only two of them (tyrosine and glutamic). Rabbits immunization with this identified T. vitulorum cross- reactive antigen in Freund's adjuvant followed by challenge with F. gigantica metacercariae resulted in 60% reduction in worm burden over control infected rabbits. Higher IgG level was detected in vaccinated rabbits four weeks post first immunization than control infected ones and remained high up to the end of the trial.