Construction of the cybrid transplastomic Brassica napus plants containing Lesquerella fendleri chloroplasts

Transferring of Lesquerella fendleri genetically transformed plastids to Brassica napus plants has been performed with the somatic hybridization method. The plastome of the previously engineered transplastomic L. fendleri plants contained the aadA16gfp selective marker gene conferring spectinomycin/...

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Published inTSitologiia i genetika Vol. 40; no. 4; p. 3
Main Authors Nitovs'ka, I O, Shakhovs'kyĭ, A M, Cherep, M N, Horodens'ka, M M, Kuchuk, M V
Format Journal Article
LanguageUkrainian
Published Ukraine 01.07.2006
Subjects
Brassica napus - genetics
Brassica napus - growth & development
Brassicaceae - genetics
Brassicaceae - growth & development
Chloroplasts - genetics
DNA, Plant - genetics
Genes, Plant
Genetic Engineering
Green Fluorescent Proteins
Plants, Genetically Modified
Polymerase Chain Reaction
Spectinomycin - pharmacology
Streptomycin - pharmacology
Transformation, Genetic
Ultraviolet Rays
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Summary:Transferring of Lesquerella fendleri genetically transformed plastids to Brassica napus plants has been performed with the somatic hybridization method. The plastome of the previously engineered transplastomic L. fendleri plants contained the aadA16gfp selective marker gene conferring spectinomycin/streptomycin resistance and green fluorescence under UV light. The protoplasts of B. napus chlorophyll-deficient plants were fused with gamma-irradiated protoplasts of L. fendleri transplastomic plants. A total of 59 green hybrid colonies have been isolated followed by spectinomycin/streptomycin selection. Shoot regeneration has been observed for two cell lines. Morphologically normal plants have been regenerated for one of them. PCR and isozyme analyses showed that the plants were transplastomic cybrids containing B. napus nuclei and L. fendleri transformed chloroplasts.
ISSN:0564-3783