Insulin degradation in hepatocytes and erythrocytes of rats in normal condition and in experimental diabetes

Insulin metabolism in erythrocytes and hepatocytes of normal and experimental diabetic rats was studied. Insulin-degrading complex of erythrocytes and hepatocytes was investigated at low insulin concentration in blood. Kinetic parameters of insulinase depend on insulin concentration in blood have sh...

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Published inVoprosy meditsinskoi khimii Vol. 47; no. 3; p. 329
Main Authors Nikolaev, S L, Strelkova, M A, Komov, V P
Format Journal Article
LanguageRussian
Published Russia (Federation) 01.05.2001
Subjects
Animals
Diabetes Mellitus, Experimental - metabolism
Erythrocytes - metabolism
Hydrolysis
Insulin - metabolism
Insulysin - metabolism
Liver - metabolism
Male
Rats
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Summary:Insulin metabolism in erythrocytes and hepatocytes of normal and experimental diabetic rats was studied. Insulin-degrading complex of erythrocytes and hepatocytes was investigated at low insulin concentration in blood. Kinetic parameters of insulinase depend on insulin concentration in blood have shown. Thermo-acid resistant non-competitive endogenous inhibitors were purified from erythrocytes and hepatocytes. Increase of endogenous inhibitors influence insulin-degrading enzyme in diabetes. Synthesis of insulin-degrading enzyme in hepatocytes of diabetic rats was reduced. These results suggest that insulin-degrading activity in erythrocytes and hepatocytes is regulated by endogenous inhibitors, by synthesis of insulinase. This change of blood insulin concentration influences practically all the components of insulin-degrading complex.
ISSN:0042-8809