mRNA expression of muscarinic receptors in spinal cord and brainstem in morphine dependent rats

• Published in: Yao hsüeh hsüeh pao Vol. 37; no. 8; p. 611
• Main Authors: Zhou, Wen-hua, Liu, Hui-fen, Gu, Jun, Xie, Xiao-hu, Tang, Shuai-en, Yang, Guo-dong, Wu, Qi-xia
• Format: Journal Article
• Language: Chinese
• Published: China 01.08.2002
• Subjects: Animals; Brain Stem - drug effects; Brain Stem - metabolism; Gene Expression - drug effects; Male; Morphine - toxicity; Morphine Dependence - metabolism; Rats; Rats, Sprague-Dawley; Receptors, Muscarinic - biosynthesis; Receptors, Muscarinic - classification; Receptors, Muscarinic - genetics; RNA, Messenger - biosynthesis; RNA, Messenger - drug effects; Spinal Cord - drug effects; Spinal Cord - metabolism; Substance Withdrawal Syndrome - metabolism
• ISSN: 0513-4870

To observe mRNA expression of muscarinic acetylcholine receptors in spinal cord and brainstem in morphine dependent or withdrawal rats. The mRNA expression level of m1, m2, m3, m4 and m5 were determined by RT-PCR, the beta-actin mRNA expression was used as internal control. The mRNA level of m1, m2, m3, m4 and m5 in spinal cord and m1 and m2 in brainstem were increased significantly during morphine dependence, and the levels of m1, m2, m3 and m4 in spinal cord and m1 in brainstem were decreased 1 h after the injection of naloxone (4 mg.kg-1, i.p.) in morphine dependent rats. Either scopolamine (0.5 mg.kg-1) or pirenzepine (10 mg.kg-1) was shown to significantly decrease the morphine withdrawal symptoms in rats. The levels of m1, m2, m3 and m5 in spinal cord were increased by pretreatment with pirenzepine and the levels of m2, m3 and m4 in spinal cord were increased by pretreatment with scopolamine. The adaptive expression of muscarinic receptors at spinal and supraspinal levels play important role in mediatin