Antibody-induced activation of beta1 integrin receptors stimulates cAMP-dependent migration of breast cells on laminin-5

The beta1 integrin-stimulating antibody TS2/16 induces cAMP-dependent migration of MCF-10A breast cells on the extracellular matrix protein laminin-5. TS2/16 stimulates a rise in intracellular cAMP within 20 min after plating. Pertussis toxin, which inhibits both antibody-induced migration and cAMP...

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Published inMolecular cell biology research communications Vol. 4; no. 2; pp. 129 - 135
Main Authors Plopper, G E, Huff, J L, Rust, W L, Schwartz, M A, Quaranta, V
Format Journal Article
LanguageEnglish
Published United States 01.08.2000
Subjects
Adenosine Diphosphate Ribose - metabolism
Antibodies, Monoclonal - pharmacology
Breast Neoplasms - pathology
Cell Adhesion
Cell Adhesion Molecules - metabolism
Cell Movement - drug effects
Cells, Cultured
Cyclic AMP - metabolism
Cyclic AMP - pharmacology
DNA Primers
Female
Heterotrimeric GTP-Binding Proteins - metabolism
Humans
Integrin alpha3beta1
Integrins - metabolism
Kalinin
Pertussis Toxin
Precipitin Tests
Receptors, Laminin - metabolism
Signal Transduction - physiology
Tumor Cells, Cultured
Virulence Factors, Bordetella - pharmacology
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Summary:The beta1 integrin-stimulating antibody TS2/16 induces cAMP-dependent migration of MCF-10A breast cells on the extracellular matrix protein laminin-5. TS2/16 stimulates a rise in intracellular cAMP within 20 min after plating. Pertussis toxin, which inhibits both antibody-induced migration and cAMP accumulation, targets the Galphai3 subunit of heterotrimeric G proteins in these cells, suggesting that Galphai3 may link integrin activation and migration via a cAMP signaling pathway.
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ISSN:1522-4724