Interaction of perch fucolectin with Lewis antigens

Interaction of fucolectin of perch Perca fluviatilis (PFL) with a set of Lewis antigens was studied by monitoring changes in its tryptophan fluorescence. PFL bound Le(c) (H type 1)-pentasaccharide (Ka = 6.6 x 10(3) M(-1)) and H type 6-trisaccharide (Ka = 2.5 x 10(3) M(-1)); essentially weaker, with...

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Bibliographic Details
Published inPrikladnaja biohimija i mikrobiologija Vol. 40; no. 4; p. 485
Main Authors Piskarev, V E, Bushueva, T L, Iamskov, I A
Format Journal Article
LanguageRussian
Published Russia (Federation) 01.07.2004
Subjects
Animals
Disaccharides - analysis
Disaccharides - chemistry
Fluorescence
Fucose - chemistry
Fucose - metabolism
Lectins - chemistry
Lectins - metabolism
Lewis Blood-Group System - analysis
Lewis Blood-Group System - chemistry
Lewis Blood-Group System - metabolism
Perches
Spectrometry, Fluorescence
Tryptophan - analysis
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Summary:Interaction of fucolectin of perch Perca fluviatilis (PFL) with a set of Lewis antigens was studied by monitoring changes in its tryptophan fluorescence. PFL bound Le(c) (H type 1)-pentasaccharide (Ka = 6.6 x 10(3) M(-1)) and H type 6-trisaccharide (Ka = 2.5 x 10(3) M(-1)); essentially weaker, with Le(b)-hexasaccharide (Ka = = 4.0 x 10(2) M(-1)); and failed to interact with Le(a)-, Le(x)-, and Le(d)-containing oligosaccharides. PFL belongs to a new type of the fucolectins recognizing H-disaccharide Fuc alpha1-2Gal within various antigens, including H type 1/2 and Le(b).
ISSN:0555-1099