Inhibitory effects of alpha-zearalenol on angiotensin II-induced integrin beta3 mRNA via suppression of nuclear factor-kappaB

• Published in: Biomedical and environmental sciences Vol. 18; no. 5; p. 314
• Main Authors: Li, Su-Min, Wang, Xiao-Ming, Qiu, Jin, Si, Qin, Guo, Heng-Yi, Sun, Ren-Yu, Wu, Qi-Xia
• Format: Journal Article
• Language: English
• Published: China 01.10.2005
• Subjects: Angiotensin II - antagonists & inhibitors; Cells, Cultured; Endothelial Cells - drug effects; Endothelial Cells - metabolism; Endothelium, Vascular - drug effects; Endothelium, Vascular - metabolism; Estradiol - pharmacology; Female; Gene Expression Regulation; Humans; Integrin beta3 - biosynthesis; Integrin beta3 - genetics; NF-kappa B - antagonists & inhibitors; NF-kappa B - physiology; Nitric Oxide - antagonists & inhibitors; Phytoestrogens - pharmacology; Receptors, Estrogen - antagonists & inhibitors; RNA, Messenger - metabolism; Zeranol - analogs & derivatives; Zeranol - pharmacology
• ISSN: 0895-3988

To investigate the effect of alpha-zearalenol on angiotensin II-induced beta3 integrin mRNA expression in human umbilical vein endothelial cells (HUVECs). The mRNA level in integrin beta3 was determined by reverse transcription-polymerase chain reaction. Endothelial NF-kappaB activity was determined by the luciferase activity assay of plasmid NF-kappaB-LUC. The angiotensin II-induced beta3 integrin mRNA expression was inhibited by alpha-zearalenol and 17beta-estradiol (10 nmol/L -1 micromol/L), but not influenced by ICI 182, 780, a pure competitive antagonist for estrogen receptor or a nitric oxide inhibitor Nomega-Nitro-L-arginine methyl ester hydrochloride. Alpha-zearalenol and 17beta-estradiol suppressed the angiotensin II-induced activation of NF-kappaB in endothelial cells. Alpha-zearalenol inhibits angiotensin II-induced integrin beta3 mRNA expression by suppressing NF-kappaB activation in endothelial cells.