Milk ultrafiltrate as a promising source of angiogenin

The use of membrane technologies in the production of soft cheese (children's food) is associated with the appearance of up to 80% of angiogenin in the ultrafiltrate. An electrophoretically homogeneous preparation of angiogenin (MW approximately 17 kDa) was obtained from milk ultrafiltrate by t...

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Bibliographic Details
Published inPrikladnaja biohimija i mikrobiologija Vol. 38; no. 2; p. 221
Main Authors Fedorova, T V, Komolova, G S, Rabinovich, M L, Tikhomirova, N A, Shalygina, A M
Format Journal Article
LanguageRussian
Published Russia (Federation) 01.03.2002
Subjects
Angiogenesis Inducing Agents - isolation & purification
Animals
Chromatography, Ion Exchange
Enzyme Stability
Food Technology
Milk - chemistry
Ribonuclease, Pancreatic - chemistry
Ribonuclease, Pancreatic - isolation & purification
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Summary:The use of membrane technologies in the production of soft cheese (children's food) is associated with the appearance of up to 80% of angiogenin in the ultrafiltrate. An electrophoretically homogeneous preparation of angiogenin (MW approximately 17 kDa) was obtained from milk ultrafiltrate by two-stage ion-exchange chromatography. The yield of the angiogenin was approximately 60%, which corresponds to a 586-fold purification of the raw material. The obtained preparation retained stability in the course of lyophilization and could be stored at 4 degrees C for a long time without decomposition.
ISSN:0555-1099