Immunomorphology in the diagnosis of orbital rhabdomyosarcoma

Immunohistochemistry (IHC) using monoclonal antibodies is presently the most reliable method to verify tumor tissue belonging. The histological diagnosis of rhabdomyosarcoma (RMS) was immunohistochemically confirmed in 7 of 10 orbital neoplasms in children. In 3 cases the diagnosis of RMS was establ...

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Bibliographic Details
Published inVestnik oftal'mologii Vol. 125; no. 2; p. 10
Main Authors Andreeva, L D, Khoroshilova-Maslova, I P
Format Journal Article
LanguageRussian
Published Russia (Federation) 01.03.2009
Subjects
Adolescent
Antibodies, Neoplasm - analysis
Biomarkers, Tumor - immunology
Biomarkers, Tumor - metabolism
Child
Child, Preschool
Diagnosis, Differential
Humans
Immunohistochemistry - methods
Infant
MyoD Protein - immunology
MyoD Protein - metabolism
Myoglobin - immunology
Myoglobin - metabolism
Orbital Neoplasms - immunology
Orbital Neoplasms - metabolism
Orbital Neoplasms - pathology
Rhabdomyosarcoma - immunology
Rhabdomyosarcoma - metabolism
Rhabdomyosarcoma - pathology
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Summary:Immunohistochemistry (IHC) using monoclonal antibodies is presently the most reliable method to verify tumor tissue belonging. The histological diagnosis of rhabdomyosarcoma (RMS) was immunohistochemically confirmed in 7 of 10 orbital neoplasms in children. In 3 cases the diagnosis of RMS was established after IHC study that revealed the atypical tumor cell expression of the muscular antigens--myoglobin, protein Myo D1, actin HHF-35, which is indicative of the rhabdomyogenic genesis of a tumor. IHC data indicated that, no matter what the histological pattern, orbital RMS is characterized by the expression of cytospecific proteins--myoglobin, Myo D1, actin HHF-35, and tissue-specific (intermediate filaments)--vimentin and desmin. The use of appropriate antibodies offers a means of making a histogenetic diagnosis even in cases of the atypical morphological pattern.
ISSN:0042-465X