A novel beta1,3-N-acetylglucosaminyltransferase (beta3Gn-T8), which synthesizes poly-N-acetyllactosamine, is dramatically upregulated in colon cancer

A new member of the UDP-N-acetylglucosamine: beta-galactose beta1,3-N-acetylglucosaminyltransferase (beta3Gn-T) family having the beta3-glycosyltransferase motifs was identified using an in silico method. This novel beta3Gn-T was cloned from a human colon cancer cell line and named beta3Gn-T8 based...

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Published inFEBS letters Vol. 579; no. 1; pp. 71 - 78
Main Authors Ishida, Hiroyasu, Togayachi, Akira, Sakai, Tokiko, Iwai, Toshie, Hiruma, Toru, Sato, Takashi, Okubo, Reiko, Inaba, Niro, Kudo, Takashi, Gotoh, Masanori, Shoda, Junichi, Tanaka, Naomi, Narimatsu, Hisashi
Format Journal Article
LanguageEnglish
Published England 03.01.2005
Subjects
Amino Acid Sequence
Cloning, Molecular
Colonic Neoplasms - enzymology
Colonic Neoplasms - genetics
Computational Biology
Gene Expression Regulation, Neoplastic
Humans
Molecular Sequence Data
N-Acetylglucosaminyltransferases - genetics
N-Acetylglucosaminyltransferases - metabolism
Polysaccharides - biosynthesis
Polysaccharides - genetics
RNA, Messenger - analysis
RNA, Messenger - metabolism
Sequence Alignment
Substrate Specificity
Transcription, Genetic
Up-Regulation
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Summary:A new member of the UDP-N-acetylglucosamine: beta-galactose beta1,3-N-acetylglucosaminyltransferase (beta3Gn-T) family having the beta3-glycosyltransferase motifs was identified using an in silico method. This novel beta3Gn-T was cloned from a human colon cancer cell line and named beta3Gn-T8 based on its position in a phylogenetic tree and enzymatic activity. Beta3Gn-T8 transfers GlcNAc to the non-reducing terminus of the Galbeta1-4GlcNAc of tetraantennary N-glycan in vitro. HCT15 cells transfected with beta3Gn-T8 cDNA showed an increase in reactivity to both LEA and PHA-L4 in a flow cytometric analysis. These results indicated that beta3Gn-T8 is involved in the biosynthesis of poly-N-acetyllactosamine chains on tetraantennary (beta1,6-branched) N-glycan. In most of the colorectal cancer tissues examined, the level of beta3Gn-T8 transcript was significantly higher than in normal tissue. Beta3Gn-T8 could be an enzyme involved in the synthesis of poly-N-acetyllactosamine on beta1-6 branched N-glycans in colon cancer.
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ISSN:0014-5793