New approach to synthesis of peptide immunomimetic of benzo[apyrene

A novel approach to discovery of the peptide with an internal immunological image of carcinogen is suggested in this work. The hybridomas producing monoclonal antibodies (mAb) B2 against benzo[a]pyrene and benz[a]antracene were prepared. Polyclonal Ab against benzo[a]pyrene (Bp) and benz[a]antracene...

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Published inMolekulârnaâ genetika, mikrobiologiâ i virusologiâ no. 3; p. 32
Main Authors Glushkov, A N, Apal'ko, S V, Filipenko, M L, Matveeva, V A, Khrapov, E A, Kostianko, M V
Format Journal Article
LanguageRussian
Published Russia (Federation) 2008
Subjects
Animals
Antibodies - blood
Antibodies - metabolism
Bacteriophages - metabolism
Benzo(a)pyrene
Carcinogens
Epitopes
Mice
Mice, Inbred ICR
Molecular Mimicry
Peptide Library
Peptides - immunology
Peptides - metabolism
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Summary:A novel approach to discovery of the peptide with an internal immunological image of carcinogen is suggested in this work. The hybridomas producing monoclonal antibodies (mAb) B2 against benzo[a]pyrene and benz[a]antracene were prepared. Polyclonal Ab against benzo[a]pyrene (Bp) and benz[a]antracene (Ba), antracene, chrysene, and pyrene were also prepared. We identified the related peptide-presenting phage specificity binding to mAT B2 and polyclonal Ab against Bp from 12 large random peptide libraries using phage display method. ICR mice were immunized with specific binding of positive phage clone for studying its immunogenicity. The Bp antibodies were found in the blood serum. All positive phage clones carried the sequence. Thus, the unique peptide with an internal immunological image of Bp may be the new candidate for anticarcinogenic vaccine.
ISSN:0208-0613