Long-term hematological reconstitution and clinical evaluation of autologous peripheral blood stem cell transplantation after cryopreservation of cells with 5% and 10% dimethylsulfoxide at -80 degrees C in a mechanical freezer

We report the long-term evaluation over 12 years of a simplified technique for stem-cell cryopreservation at -80 degrees C without rate-controlled freezing and with 5% (n=251) or 10% (n=47) DMSO as the sole cryoprotectant. Platelet recovery was greater in the 5% DMSO group while long-term hematologi...

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Bibliographic Details
Published inHaematologica (Roma) Vol. 92; no. 7; p. 986
Main Authors Galmes, Antonio, Gutiérrez, Antonio, Sampol, Antonia, Canaro, Mariana, Morey, Miguel, Iglesias, Julio, Matamoros, Nuria, Duran, María Antonia, Novo, Andrés, Bea, María Dolores, Galán, Pilar, Balansat, Josep, Martínez, Jordi, Bargay, Joan, Besalduch, Joan
Format Journal Article
LanguageEnglish
Published Italy 01.07.2007
Subjects
Blood Preservation - methods
Cryopreservation - methods
Cryoprotective Agents - pharmacology
Dimethyl Sulfoxide - pharmacology
Hematopoiesis - drug effects
Hematopoietic Stem Cells - cytology
Humans
Peripheral Blood Stem Cell Transplantation
Transplantation, Autologous
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Summary:We report the long-term evaluation over 12 years of a simplified technique for stem-cell cryopreservation at -80 degrees C without rate-controlled freezing and with 5% (n=251) or 10% (n=47) DMSO as the sole cryoprotectant. Platelet recovery was greater in the 5% DMSO group while long-term hematological recovery did not differ. Factors influencing a faster hematological recovery were infusion of more than 2.7x10(6)/Kg of CD34+ cells, 10% DMSO cryopreservation and G-CSF. We confirm that the procedure is feasible with reduction in infusion-related toxicity from 60% using 5% DMSO. Differences in hematological reconstitution were not clinically significant if a minimum of 1.5x10(6)/Kg CD34+-cells were infused.
ISSN:1592-8721