Experimental study of cytokines complex inhibitory effect on wound healing after glaucoma filtering operations: histopathological and immunochemical findings

Experiments on a model of glaucoma filtering operation in 25 rabbits showed that the natural cytokine complex inhibited the operation wound healing in comparison with the control. The complex was prepared by an original method (culturing peripheral blood leukocytes) at Immunology Department of the R...

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Published inVestnik oftal'mologii Vol. 116; no. 1; p. 5
Main Authors Khoroshilova-Maslova, I P, Gankovskaia, L V, Andreeva, L D, Erichev, V P, Vasilenkova, L V, Ilatovskaia, L V
Format Journal Article
LanguageRussian
Published Russia (Federation) 01.01.2000
Subjects
Animals
Anterior Chamber - immunology
Anterior Chamber - pathology
Anterior Chamber - surgery
Cicatrix - immunology
Cicatrix - metabolism
Cicatrix - pathology
Cicatrix - prevention & control
Collagen - metabolism
Conjunctiva - immunology
Conjunctiva - pathology
Conjunctiva - surgery
Cytokines - pharmacology
Disease Models, Animal
Filtering Surgery
Glaucoma - surgery
Rabbits
Wound Healing - drug effects
Wound Healing - immunology
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Summary:Experiments on a model of glaucoma filtering operation in 25 rabbits showed that the natural cytokine complex inhibited the operation wound healing in comparison with the control. The complex was prepared by an original method (culturing peripheral blood leukocytes) at Immunology Department of the Russian State Medical University. Histopathological study revealed delayed formation of cicatricial tissue under the effect of cytokines. Decreased proliferation of fibroblasts and their decreased functional activity (decreased synthetic activity of glycosaminoglycanes and collagen) was detected in cicatricial tissue. Immunohistochemical studies showed that in remote periods (after 3-8 months) type IV membrane collagen formed in new cicatricial tissue, while in the control, types III and I collagens predominated in cicatricial tissue. Immunohistochemical structure of the cicatrix in experiment correlated with fine fibrous structure of cicatricial tissue, characterized by high permeability. Possible mechanisms of the inhibitory effect of the natural cytokine complex on the course of operation wound healing after glaucoma-filtering surgery are discussed.
ISSN:0042-465X