Cytokine system in patients with chronic hepatitis C during treatment with interferon-alfa

To study changes in serum levels of interleukine-1 beta (IL-1b), IL-6, TNF-alpha (TNFa), HM-HMF and TFR-1 beta (TFR-1b), expression of surface antigens CD14 and CD95 on blood monocytes from patients with chronic hepatitis C (CHC) treated with interferon-alpha (INFa). Examinations covered 25 CHC pati...

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Published inTerapevtic̆eskii arhiv Vol. 74; no. 2; p. 37
Main Authors Ivashkin, V T, Mammaev, S N, Lukina, E A, Lugovskaia, S A, Shul'pekova, Iu O, Levina, A A, Pochtar', M E
Format Journal Article
LanguageRussian
Published Russia (Federation) 2002
Subjects
Adolescent
Adult
Antiviral Agents - therapeutic use
CD4 Antigens - blood
fas Receptor - blood
Female
Hepatitis C - drug therapy
Hepatitis C - immunology
Humans
Interferon-alpha - therapeutic use
Interleukin-1 - blood
Interleukin-6 - blood
Lymphotoxin-alpha - blood
Male
Middle Aged
Monocytes - metabolism
Recombinant Proteins
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Summary:To study changes in serum levels of interleukine-1 beta (IL-1b), IL-6, TNF-alpha (TNFa), HM-HMF and TFR-1 beta (TFR-1b), expression of surface antigens CD14 and CD95 on blood monocytes from patients with chronic hepatitis C (CHC) treated with interferon-alpha (INFa). Examinations covered 25 CHC patients and 25 healthy controls. Concentrations of proinflammatory cytokines and growth factors in blood serum were measured with ELISA (kits by "R&D systems", USA). CD14 and CD95 antigen expression on monocytes of venous blood were studied using flow cytoflowmeter (Partes, USA) before and after a 12-week course of INFa. Before INFa treatment CHC patients had significantly elevated serum concentrations of TNFa, HM-KSF and TFR-1b. Coexpression of antigens CD14+ and CD95+ was found on 61% of blood monocytes. Three-month INFa treatment lowered levels of TNFa, GM-KSF and CD95+ expression on monocytes as well as TFR-1b concentration in the serum which correlated with a positive trend in the standard clinicolaboratory and virusological indices in the examinees. Changes in serum indices of proinflammatory cytokines and growth factors, in expression of CD95 on blood monocytes from CHC patients treated with INFa show an important role of cytokines system activation and mechanisms of programmed cell death in pathogenesis of chronic HCV infection.
ISSN:0040-3660