Contrast staining of Trichinella spiralis larvae in fresh, frozen and formalin preserved muscles

Fresh, frozen-thawed and formalin-preserved muscle samples heavily infected with Trichinella larvae were cut into several pieces and stained with Giemsa and Leishman and the reference Haematoxylineosin (H & E) stain. Observation under microscope revealed that both muscle larvae and nurse cells i...

Full description

Saved in:
Bibliographic Details
Published inJournal of the Egyptian Society of Parasitology Vol. 37; no. 3; p. 1031
Main Authors Ali, Nehad M, Thabet, Hala S
Format Journal Article
LanguageEnglish
Published Egypt 01.12.2007
Subjects
Animals
Azure Stains
Female
Larva
Muscles - parasitology
Rats
Specimen Handling - methods
Trichinella spiralis - isolation & purification
Trichinella spiralis - ultrastructure
Trichinellosis - diagnosis
Trichinellosis - parasitology
Online AccessGet more information

Cover

More Information
Summary:Fresh, frozen-thawed and formalin-preserved muscle samples heavily infected with Trichinella larvae were cut into several pieces and stained with Giemsa and Leishman and the reference Haematoxylineosin (H & E) stain. Observation under microscope revealed that both muscle larvae and nurse cells in fresh and formalin preserved specimens appeared as purplish blue structures contrasting with the pinkish color of non-infected muscle fibers in both Giemsa and Leishman stains. These findings were confirmed in H & E stained samples. However, frozen samples did not show contrast stain.
ISSN:1110-0583