Effects of green tea extract on expression of human papillomavirus type 16 oncoproteins-induced hypoxia-inducible factor-1alpha and vascular endothelial growth factor in human cervical carcinoma cells

• Published in: Zhong hua yi xue za zhi Vol. 88; no. 40; p. 2872
• Main Authors: Tang, Xu-dong, Zhou, Xin, Zhang, Qun-zhou, Le, Anh D, Zhou, Ke-yuan
• Format: Journal Article
• Language: Chinese
• Published: China 04.11.2008
• Subjects: Catechin - pharmacology; Cell Line, Tumor; Female; Gallic Acid - pharmacology; Human papillomavirus 16; Humans; Hypoxia-Inducible Factor 1, alpha Subunit - biosynthesis; Plant Extracts - pharmacology; Tea; Uterine Cervical Neoplasms - metabolism; Uterine Cervical Neoplasms - virology; Vascular Endothelial Growth Factor A - biosynthesis
• ISSN: 0376-2491

To investigate the effects of green tea extract (GTE) and its major component (-)-epigallocatechin-3-gallate (EGCG) on the human papillomavirus (HPV) type 16 oncoproteins (E6 and E7)-induced expression of hypoxia-inducible factor-1alpha (HIF-1alpha) and vascular endothelial growth factor (VEGF) in human cervical carcinoma cells. Human cervical carcinoma cells of the line C-33A were divided to 4 groups to be transiently transfected with the plasmid HA-pSG5-HPV-16E6 containing the HPV type 16 oncoprotein E6, HA-pSG5-HPV-16 E7, or empty plasmid HA-pSG5, or just exposed to the transfection reagent Lipofectamine 2000 as mock transfection control group. Western blotting and ELISA were used to detect the protein expression of HIF-1alpha and the protein expression of VEGF 18, 24, and 48 h after the transfection. Twenty-four hours after the transfection, the transfected-cells were treated with GTE (40 microg/ml) or EGCG (50 micromol/L) for 8, 16, and 24 h respectively, or treated with GTE of the concentrations of 20,