Effect of 5-HT(2) receptor blockade on cadmium-induced acute toxicity

The protective effect of 5-HT(2) receptor blockade with ketanserin or ritanserin against cadmium liver injury was investigated. Male Wistar rats were injected intraperitoneally with a sublethal dose of cadmium (3.5 mg/kg body weight). Rats were treated with normal saline (group I), ketanserin (3 mg/...

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Published inDigestive diseases and sciences Vol. 52; no. 9; p. 2351
Main Authors Tzirogiannis, Konstantinos N, Demonakou, Maria D, Papadimas, George K, Skaltsas, Spyridon D, Manta, Georgia A, Kourentzi, Kalliopi T, Alexandropoulou, Katerina N, Hereti, Rosa I, Mykoniatis, Michael G, Panoutsopoulos, Georgios I
Format Journal Article
LanguageEnglish
Published United States 01.09.2007
Subjects
Animals
Apoptosis - drug effects
Cadmium - toxicity
Disease Models, Animal
In Situ Nick-End Labeling
Ketanserin - therapeutic use
Liver Failure, Acute - chemically induced
Liver Failure, Acute - drug therapy
Liver Failure, Acute - metabolism
Male
Rats
Rats, Wistar
Receptors, Serotonin, 5-HT2 - metabolism
Ritanserin - therapeutic use
Serotonin 5-HT2 Receptor Antagonists
Serotonin Antagonists - therapeutic use
Treatment Outcome
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Summary:The protective effect of 5-HT(2) receptor blockade with ketanserin or ritanserin against cadmium liver injury was investigated. Male Wistar rats were injected intraperitoneally with a sublethal dose of cadmium (3.5 mg/kg body weight). Rats were treated with normal saline (group I), ketanserin (3 mg/kg body weight; group II), or ritanserin (3 mg/kg body weight; group III) 2 hr prior and 4 hr after cadmium administration and killed at different time points. Hematoxylin/eosin-stained liver sections were assessed for necrosis, apoptosis, peliosis, mitoses, and inflammatory infiltration. Apoptosis was also quantified by the TUNEL assay. Nonparenchymal liver cells and activated Kupffer cells were identified histochemically. Necrosis, hepatocyte apoptosis, nonparenchymal cell apoptosis, and macroscopic and microscopic peliosis were markedly reduced or minimized in ketanserin- or ritanserin-treated rats. The observed protective effect was almost identical for both ketanserin and ritanserin administration. 5-HT(2) receptor blockade exerts a protective effect against acute cadmium-induced hepatotoxicity.
ISSN:0163-2116