Use of redox polymers for insulin labeling with technetium (99mTc)

The paper describes a method for porcine insulin labelling with technetium(99mTc). When preparing this peptide, an insoluble redox polymer on the basis of cross-linked dextran (RP G25-IDA) was used for labelling. The prepared kits were labelled with 99mTc with a high labelling efficiency (more than...

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Bibliographic Details
Published inČeská a Slovenská farmacie Vol. 48; no. 5; p. 218
Main Authors Komárek, P, Kleisner, I, Konopková, M, Komárková, I
Format Journal Article
LanguageCzech
Published Czech Republic 01.09.1999
Subjects
Animals
Female
Insulin - pharmacokinetics
Isotope Labeling - methods
Male
Oxidation-Reduction
Polymers
Rats
Rats, Wistar
Technetium
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Summary:The paper describes a method for porcine insulin labelling with technetium(99mTc). When preparing this peptide, an insoluble redox polymer on the basis of cross-linked dextran (RP G25-IDA) was used for labelling. The prepared kits were labelled with 99mTc with a high labelling efficiency (more than 95%) and stability, as assessed using methods of chromatography. 99mTc-insulin biodistribution in rats with experimentally induced diabetes--compared with a control group--did not demonstrate specific accumulation of the labelled substance in any organ. The method for kit preparation and its labelling are mild, HPLC and SEC did not reveal any products of insulin degradation and insulin content met the standards of Ph.B. 97. This is the first case of insulin labelling with technetium(99Tc) and, also, the first use of a redox polymer for this purpose.
ISSN:1210-7816