Lipid peroxidation and nitric oxide inactivation in postmenopausal women

To assess the effect of endogenous estrogens on the bioavailability of nitric oxide (.NO) and in the formation of lipid peroxidation products in pre- and postmenopausal women. NOx and S-nitrosothiols were determined by gaseous phase chemiluminescence, nitrotyrosine was determined by ELISA, COx (chol...

Full description

Saved in:
Bibliographic Details
Published inArquivos brasileiros de cardiologia Vol. 80; no. 4; pp. 406 - 423
Main Authors Pereira, Isabela Rosier Olimpio, Bertolami, Marcelo Chiara, Faludi, André Arpad, Campos, Maria Fernanda, Ferderbar, Simone, Lima, Emersom Silva, Aldrighi, José Mendes, Abdalla, Dulcineia Saes Parra
Format Journal Article
LanguageEnglish
Portuguese
Published Brazil 01.04.2003
Subjects
Adult
Aged
Arteriosclerosis - etiology
Cholesterol - blood
Estradiol - blood
Estrogens - physiology
Female
Humans
Lipid Peroxidation
Middle Aged
Nitric Oxide - blood
Nitric Oxide - metabolism
Postmenopause - blood
Postmenopause - metabolism
Premenopause - blood
Premenopause - metabolism
S-Nitrosothiols - blood
Tyrosine - analogs & derivatives
Tyrosine - blood
Vasodilation
Online AccessGet full text

Cover

More Information
Summary:To assess the effect of endogenous estrogens on the bioavailability of nitric oxide (.NO) and in the formation of lipid peroxidation products in pre- and postmenopausal women. NOx and S-nitrosothiols were determined by gaseous phase chemiluminescence, nitrotyrosine was determined by ELISA, COx (cholesterol oxides) by gas chromatography, and cholesteryl linoleate hydroperoxides (CE18:2-OOH), trilinolein (TG18:2-OOH), and phospholipids (PC-OOH) by HPLC in samples of plasma. The concentrations of NOx, nitrotyrosine, COx, CE18:2-OOH, and PC-OOH were higher in the postmenopausal period (33.8+/-22.3 microL; 230+/-130 nM; 55+/-19 ng/microL; 17+/-8.7 nM; 2775+/-460 nM, respectively) as compared with those in the premenopausal period (21.1+/-7.3 microM; 114+/-41 nM; 31+/-13 ng/microL; 6+/-1.4 nM; 1635+/-373 nM). In contrast, the concentration of S-nitrosothiols was lower in the postmenopausal period (91+/-55 nM) as compared with that in the premenopausal p in the premenopausal period (237+/-197 nM). In the postmenopausal period, an increase in nitrotyrosine and a reduction of S-nitrosothiol formation, as well as an increase of COx, CE18:2-OOH and PC-OOH formation occurs. Therefore, NO inactivation and the increase in lipid peroxidation may contribute to endothelial dysfunction and to the greater risk for atherosclerosis in postmenopausal women.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0066-782X