Identification of a novel allele human leukocyte antigen B4609

To identify a novel human leukocyte antigen (HLA) allele. HLA typing was carried out with PCR-SSOP. Molecular cloning and DNA sequencing were used to identify the sequence of a potential novel allele and the difference between this new allele and other known alleles was analyzed. HLA genotyping of o...

Full description

Saved in:
Bibliographic Details
Published inZhonghua yi xue yi chuan xue za zhi Vol. 25; no. 4; p. 459
Main Authors Chen, Yang, Li, Jian-ping, Zhang, Kun-lian, Zhang, Xu, Liu, Xian-zhi
Format Journal Article
LanguageChinese
Published China 01.08.2008
Subjects
Alleles
Amino Acid Substitution
Base Sequence
Cloning, Molecular
HLA Antigens - genetics
HLA Antigens - immunology
HLA-B Antigens - genetics
HLA-B Antigens - immunology
Humans
Molecular Sequence Data
Polymorphism, Single Nucleotide
Sequence Homology, Nucleic Acid
Online AccessGet more information

Cover

More Information
Summary:To identify a novel human leukocyte antigen (HLA) allele. HLA typing was carried out with PCR-SSOP. Molecular cloning and DNA sequencing were used to identify the sequence of a potential novel allele and the difference between this new allele and other known alleles was analyzed. HLA genotyping of one sample gave different results. The sequencing results showed that the HLA B alleles of the proband were B*151101 and a novel allele. The nucleotide sequence of the novel allele was different from all other known B alleles. It had one nucleotide change from the closest matching allele B*460101 at nucleotide 527 (A to T) in exon 3, resulting in an amino acid change from E (GAG) to V (GTG) at codon 176. A novel HLA B allele was identified and officially designated as HLA B*4609 by WHO Nomenclature Committee for Factors of the HLA System in November, 2006.
ISSN:1003-9406