Piceatannol attenuates lipopolysaccharide-induced NF-kappaB activation and NF-kappaB-related proinflammatory mediators in BV2 microglia

Proinflammatory mediators, such as prostaglandin E(2) (PGE(2)), nitric oxide (NO), and proinflammatory cytokines [interlukin (IL)-1beta, IL-6, and tumor necrosis factor (TNF)-alpha] play pivotal roles in brain injuries. Anti-inflammatory responses are associated with significant downregulation of th...

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Published inPharmacological research Vol. 54; no. 6; pp. 461 - 467
Main Authors Jin, Cheng-Yun, Moon, Dong-Oh, Lee, Kyeong-Jun, Kim, Mun-Ok, Lee, Jae-Dong, Choi, Yung Hyun, Park, Yeong-Min, Kim, Gi-Young
Format Journal Article
LanguageEnglish
Published Netherlands 01.12.2006
Subjects
Animals
Blotting, Western
Cell Line
Cell Survival - drug effects
Cyclooxygenase 2 - biosynthesis
Cytokines - biosynthesis
Dinoprostone - biosynthesis
Dose-Response Relationship, Drug
Enzyme Inhibitors - pharmacology
Enzyme-Linked Immunosorbent Assay
Inflammation Mediators - pharmacology
Lipopolysaccharides - pharmacology
Luciferases - biosynthesis
Mice
Microglia - drug effects
NF-kappa B - drug effects
NF-kappa B - physiology
Nitric Oxide - biosynthesis
Nitric Oxide Synthase Type II - biosynthesis
Nitrites - analysis
Nitrites - metabolism
Reverse Transcriptase Polymerase Chain Reaction
RNA - biosynthesis
Stilbenes - pharmacology
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Summary:Proinflammatory mediators, such as prostaglandin E(2) (PGE(2)), nitric oxide (NO), and proinflammatory cytokines [interlukin (IL)-1beta, IL-6, and tumor necrosis factor (TNF)-alpha] play pivotal roles in brain injuries. Anti-inflammatory responses are associated with significant downregulation of these proinflammatory mediators following brain injury. In the present study, we investigated the effects of piceatannol (PIC) on the production of proinflammatory mediators in lipopolysaccharide (LPS)-stimulated BV2 microglia. PIC significantly inhibited the release of NO, PGE(2), and proinflammatory cytokines in a dose-dependent manner. PIC also attenuated the expression of inducible NO synthase (iNOS) and cyclooxygenase (COX)-2 mRNA and protein levels. Moreover, PIC prevented NF-kappaB p65 nuclear translocation. Our data also indicate that PIC exhibits anti-inflammatory properties by suppressing the transcription of proinflammatory cytokine genes through the NF-kappaB signaling pathway. The anti-inflammatory properties of PIC may be useful for attenuating inflammatory diseases and LPS-stimulated microglial activation.
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ISSN:1043-6618