Immunization with fibrillar Abeta(1-42) in young and aged canines: Antibody generation and characteristics, and effects on CSF and brain Abeta

• Published in: Vaccine Vol. 24; no. 15; pp. 2824 - 2834
• Main Authors: Head, Elizabeth, Barrett, Edward G, Murphy, M Paul, Das, Pritam, Nistor, Mihaela, Sarsoza, Floyd, Glabe, Charles C, Kayed, Rakez, Milton, Saskia, Vasilevko, Vitaly, Milgram, Norton W, Agadjanyan, Michael G, Cribbs, David H, Cotman, Carl W
• Format: Journal Article
• Language: English
• Published: Netherlands 05.04.2006
• Subjects: Adjuvants, Immunologic - administration & dosage; Alum Compounds - administration & dosage; Alzheimer Disease - immunology; Alzheimer Disease - prevention & control; Alzheimer Vaccines - administration & dosage; Alzheimer Vaccines - immunology; Amyloid beta-Peptides - administration & dosage; Amyloid beta-Peptides - cerebrospinal fluid; Amyloid beta-Peptides - immunology; Animals; Cerebral Cortex - metabolism; Dogs; Enzyme-Linked Immunosorbent Assay; Epitope Mapping; Female; Histocytochemistry; Humans; Immunoglobulin G - blood; Immunoglobulin M - blood; Male; Models, Animal; Peptide Fragments - administration & dosage; Peptide Fragments - immunology; Poloxalene - administration & dosage
• ISSN: 0264-410X

We describe a study testing fibrillar beta-amyloid(1-42) (Abeta42) vaccination in dogs. Three young beagles (4.6 years) were immunized twice with Abeta42 and a Th1 adjuvant (TiterMax Gold). Animals generated primarily IgG2 and IgM antibody responses, which were specific for the Abeta(11-30) region of Abeta(1-42). Next, 3 aged beagles (8.9-13.8 years) were immunized 4 times with Abeta(42) and a Th2 adjuvant (Alum). We observed an acute increase in IgG2, a slower increase in IgG1 and Abeta antibodies of broader specificity (Abeta(1-15>) Abeta(11-30>) Abeta(6-20)). A nonsignificant increase in CSF Abeta(1-40) and decrease in Abeta(1-40/1-42) in cortex was detected. Canines may be a useful system for testing an Abeta vaccine.