Lipid phosphate phosphatase-1 regulates lysophosphatidic acid-induced calcium release, NF-kappaB activation and interleukin-8 secretion in human bronchial epithelial cells

• Published in: Biochemical journal Vol. 385; no. Pt 2; pp. 493 - 502
• Main Authors: Zhao, Yutong, Usatyuk, Peter V, Cummings, Rhett, Saatian, Bahman, He, Donghong, Watkins, Tonya, Morris, Andrew, Spannhake, Ernst W M, Brindley, David N, Natarajan, Viswanathan
• Format: Journal Article
• Language: English
• Published: England 15.01.2005
• Subjects: Adenoviridae; Arginine - genetics; Arginine - physiology; Bronchi - cytology; Calcium - metabolism; Cell Extracts - chemistry; Cells, Cultured; Epithelial Cells - enzymology; Epithelial Cells - metabolism; Epithelial Cells - secretion; Epithelial Cells - virology; Humans; Interleukin-8 - metabolism; Interleukin-8 - secretion; Lung Transplantation; Lysine - genetics; Lysine - physiology; Lysophospholipids - metabolism; Mutation, Missense - genetics; Mutation, Missense - physiology; NF-kappa B - metabolism; Phosphatidate Phosphatase - biosynthesis; Phosphatidate Phosphatase - genetics; Phosphatidate Phosphatase - physiology; Receptors, Lysophosphatidic Acid - agonists; Tissue Donors
• ISSN: 1470-8728

LPA (lysophosphatidic acid), a potent bioactive phospholipid, elicits diverse cellular responses through activation of the G-protein-coupled receptors LPA1-LPA4. LPA-mediated signalling is partially regulated by LPPs (lipid phosphate phosphatases; LPP-1, -2 and -3) that belong to the phosphatase superfamily. This study addresses the role of LPPs in regulating LPA-mediated cell signalling and IL-8 (interleukin-8) secretion in HBEpCs (human bronchial epithelial cells). Reverse transcription-PCR and Western blotting revealed the presence and expression of LPP-1-3 in HBEpCs. Exogenous [3H]oleoyl LPA was hydrolysed to [3H]-mono-oleoylglycerol. Infection of HBEpCs with an adenoviral construct of human LPP-1 for 48 h enhanced the dephosphorylation of exogenous LPA by 2-3-fold compared with vector controls. Furthermore, overexpression of LPP-1 partially attenuated LPA-induced increases in the intracellular Ca2+ concentration, phosphorylation of IkappaB (inhibitory kappaB) and translocation of NF-kappaB (nuclear factor-kappaB) to the nucleus, and almost completely prevented IL-8 secretion. Infection of cells with an adenoviral construct of the mouse LPP-1 (R217K) mutant partially attenuated LPA-induced IL-8 secretion without altering LPA-induced changes in intracellular Ca2+ concentration, phosphorylation of IkappaB, NF-kappaB activation or IL-8 gene expression. Our results identify LPP-1 as a key regulator of LPA signalling and IL-8 secretion in HBEpCs. Thus LPPs could represent potential targets in regulating leucocyte infiltration and airway inflammation.