Adhesion, proliferation and osteodifferentiation of bone mesenchymal stem cells on PLGA-[ASP-PEGtri-bolck polymer scaffolds

• Published in: Zhongguo gu shang Vol. 21; no. 4; p. 282
• Main Authors: Duan, Zhi-xia, Zheng, Qi-xin, Guo, Xiao-dong, Bai, Yu, Yuan, Quan, Chen, Shun-guang
• Format: Journal Article
• Language: Chinese
• Published: China 01.04.2008
• Subjects: Animals; Aspartic Acid - chemistry; Bone and Bones - cytology; Cell Adhesion; Cell Differentiation; Cell Proliferation; Female; Lactic Acid - chemistry; Male; Mesenchymal Stromal Cells - cytology; Polyethylene Glycols - chemistry; Polyglycolic Acid - chemistry; Rats; Rats, Sprague-Dawley; Tissue Engineering
• ISSN: 1003-0034

To explore the adhesion,proliferation and osteodifferentiation of bone mesenchymal stem cells (BMSCs)on the prepared lactic acid/glycolic acid/asparagic acid-co-polyethylene glycol(PLGA-[ASP-PEG])tri-block polymer scaffolds. Modified PLGA with polyethylene glycol (PEG) and asparagic acid(ASP)that has many liga nds,and then the synthesis PLGA-[ASP-PEG] tri-block polymer material was prepared. BMSCs were cultured in PLGA-[ASP-PEG] polymer material and poly lactic acid-co-glycolic acid(PLGA)were used as control group. Precipitation method, MUT assay and total cellular protein detection were used to test the adhersion and proliferation of BMSCs. After the third generation of BMSCs was cultured on PLGA-[ASP-PEG] tri-block polymer scaffolds for 14 day and 28 day with osteogenic supplements,the osteodifferentiation of MSCs were observed through alkaline phosphatase(ALP) staining and calcium tubercle staining. BMSCs grew adherent to the surface of PLGA-[ASP-PEG] polymer scaffolds and the number of BMSCs was much high