Enzymatic methods for quantitative determination of total cholesterol in blood serum

• Published in: Voprosy meditsinskoi khimii Vol. 37; no. 4; p. 85
• Main Authors: Pedchenko, V V, Malakhov, V N
• Format: Journal Article
• Language: Russian
• Published: Russia (Federation) 01.07.1991
• Subjects: Cholesterol - blood; Cholesterol Esters - blood; Cholesterol Oxidase - metabolism; Clinical Enzyme Tests; Enzymes, Immobilized; Humans; Sterol Esterase - metabolism

Advantages and disadvantages of enzymatic assays of total cholesterol content in human blood serum are reviewed. Factors, affecting these assays are discussed: specificity of the enzymes used (cholesterol esterase and cholesterol oxidase), conditions of analysis and composition of the reaction mixtu...