Enzymatic methods for quantitative determination of total cholesterol in blood serum

Advantages and disadvantages of enzymatic assays of total cholesterol content in human blood serum are reviewed. Factors, affecting these assays are discussed: specificity of the enzymes used (cholesterol esterase and cholesterol oxidase), conditions of analysis and composition of the reaction mixtu...

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Bibliographic Details
Published inVoprosy meditsinskoi khimii Vol. 37; no. 4; p. 85
Main Authors Pedchenko, V V, Malakhov, V N
Format Journal Article
LanguageRussian
Published Russia (Federation) 01.07.1991
Subjects
Cholesterol - blood
Cholesterol Esters - blood
Cholesterol Oxidase - metabolism
Clinical Enzyme Tests
Enzymes, Immobilized
Humans
Sterol Esterase - metabolism
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Summary:Advantages and disadvantages of enzymatic assays of total cholesterol content in human blood serum are reviewed. Factors, affecting these assays are discussed: specificity of the enzymes used (cholesterol esterase and cholesterol oxidase), conditions of analysis and composition of the reaction mixture, interference with components of blood serum. Adaptation of the enzymatic assays of total cholesterol estimation for various types of biochemical analyzers, including systems containing immobilized enzymes, is considered.
ISSN:0042-8809