Erythrocytic immunoreagents for diagnosing autoimmune disorders of the pancreas

Amidol sensitization of formalin-treated sheep red blood cells was found to be the optimal method for the preparation of an erythrocytic diagnostic agent from the summary antigen of the pancreas. Organ specificity of serum antibodies detection with the use of the developed diagnostic agent was provi...

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Bibliographic Details
Published inLaboratornoe delo no. 11; p. 44
Main Authors Kim, K V, Deriabin, P N, Karal'nik, B V
Format Journal Article
LanguageRussian
Published Russia (Federation) 1991
Subjects
Autoimmune Diseases - diagnosis
Autoimmune Diseases - immunology
Erythrocytes - immunology
Humans
Pancreatic Diseases - diagnosis
Pancreatic Diseases - immunology
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Summary:Amidol sensitization of formalin-treated sheep red blood cells was found to be the optimal method for the preparation of an erythrocytic diagnostic agent from the summary antigen of the pancreas. Organ specificity of serum antibodies detection with the use of the developed diagnostic agent was provided by preliminary adsorption of the tested sera with rhesus-positive human red cells, group IV (AB). Use of this diagnostic agent in the neutralization test permitted a highly specific detection of the pancreatic antigen. Antigen-specific lymphocytes detectable in the indirect rosette-formation test with the erythrocytic diagnostic agent from the pancreatic summary antigen, based on chicken red cells were found highly specific.
ISSN:0023-6748