Feasibility of using human spleen cells to produce interferon

Spleen cells of subjects who died suddenly are capable of producing large amounts of interferon in response to induction with Newcastle disease virus (NDV) and Sendai virus (1-2.5 IU per 1000 cells). The optimal conditions for interferon production by these cells include using NDV, the H strain, as...

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Bibliographic Details
Published inVoprosy virusologiĭ no. 1; p. 64
Main Authors Orlova, T G, Berulava, I I, Mentkevich, L M, Shcheglovitova, O N, Kulagin, I N
Format Journal Article
LanguageRussian
Published Russia (Federation) 01.01.1981
Subjects
Cold Temperature
Humans
In Vitro Techniques
Interferon Inducers
Interferons - biosynthesis
Newcastle disease virus
Parainfluenza Virus 1, Human
Spleen - metabolism
Tissue Preservation - methods
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Summary:Spleen cells of subjects who died suddenly are capable of producing large amounts of interferon in response to induction with Newcastle disease virus (NDV) and Sendai virus (1-2.5 IU per 1000 cells). The optimal conditions for interferon production by these cells include using NDV, the H strain, as an inducer in a dose of 10 ID50/cell and fresh spleen cells stored no more than 24 hours at 4-6 degrees C in a concentration of 0.5-1.0 x 10(7)/ml, with the incubation time of 20-24 hours. It is recommended that spleen cells from subjects who died suddenly be used for human interferon production.
ISSN:0507-4088