Proteolytic enzymes from Streptomyces fradiae: a metalloendopeptidase, subtilisin-like, and trypsin-like proteinases

• Published in: Biokhimiia (Moscow, Russia) Vol. 61; no. 2; p. 344
• Main Authors: Bormatova, M E, Ivanova, N M, Iusupova, M P, Voiushina, T L, Surova, I A, Chestukhina, G G, Stepanov, V M
• Format: Journal Article
• Language: Russian
• Published: Russia (Federation) 01.02.1996
• Subjects: Amino Acid Sequence; Chromatography, Liquid; Enzyme Stability; Hot Temperature; Hydrogen-Ion Concentration; Hydrolysis; Metalloendopeptidases - chemistry; Metalloendopeptidases - isolation & purification; Metalloendopeptidases - metabolism; Molecular Sequence Data; Sequence Homology, Amino Acid; Serine Endopeptidases - chemistry; Serine Endopeptidases - isolation & purification; Serine Endopeptidases - metabolism; Streptomyces - enzymology; Subtilisins - chemistry; Subtilisins - metabolism; Trypsin - chemistry; Trypsin - metabolism
• ISSN: 0320-9725

Three proteolytic enzymes-the metalloproteinase, SFMP, and two serine proteinases, SFSP and SFTP-have been isolated and purified from the culture fluid of Streptomyces fradiae using chromatography on bacitracin-silochrome, bacitracin-Sepharose, DEAE-cellulose and fractionation by ammonium sulfate. Study of physico-chemical and functional properties of the enzymes and structural analysis revealed that SFMP is a cysteine-containing metalloendopeptidase with M(r) of 36 kDa, has a peak activity for synthetic substrates at pH 7.0-7.5 and at 60-65 degrees C and is stable at pH 7.0-9.0. The serine proteinase SFSP is related to subtilisin-like enzymes, has a M(r) of 29 kDa and a pH optimum at 7.5-8.5 at temperature up to 50 degrees C. The proteinase is stable at pH 4.0-9.0 and retains 30% of its activity at 70 degrees C. The other serine proteinase, SFTP, has a M(r) of 26 kDa and is related to trypsin-like enzymes. Its activity for synthetic substrates of trypsin is maximal at pH 6.8-8.8 at 50 degrees C. The enzyme is stable at pH 4.5-8.5 and at temperature below 50 degrees C. It has been shown that Streptomyces fradiae, like Streptomyces griseus and other Streptomycetes, possesses an ability to secrete serine proteinases (SFSP and SFTP) related to two evolutionally distinct families of serine proteinases, i.e., subtilisin and chymotrypsin families. SFMP and SFSP have been isolated and characterized for the first time.