Quantitative determination of total mitochondrial protein with Coomassie

The mechanism of Coomassie interaction with proteins was being studied. Under certain conditions the range of detectible polypeptides can be wider. For protein analysis in mitochondria we have suggested a new component ratio for the Bradford reagent. A correction for mitochondrial protein was determ...

Full description

Saved in:
Bibliographic Details
Published inPrikladnaja biohimija i mikrobiologija Vol. 20; no. 6; p. 823
Main Authors Aliverdieva, D A, Shol'ts, K F
Format Journal Article
LanguageRussian
Published Russia (Federation) 01.11.1984
Subjects
Animals
Drug Interactions
Indicators and Reagents
Methods
Mitochondria, Liver - analysis
Proteins - analysis
Proteins - isolation & purification
Rats
Rosaniline Dyes - pharmacology
Online AccessGet more information

Cover

More Information
Summary:The mechanism of Coomassie interaction with proteins was being studied. Under certain conditions the range of detectible polypeptides can be wider. For protein analysis in mitochondria we have suggested a new component ratio for the Bradford reagent. A correction for mitochondrial protein was determined, which should be made on using bovine serum albumin as a standard.
ISSN:0555-1099