Extractive polarographic method of controlling the stereospecific hydrolysis of N-acylated derivatives of amino acid racemates

The equilibrium distribution of acetyl-D-phenylglycine prepared with stereospecific hydrolysis of acetyl-D,L-phenylglycine (Ac-D,L-PhG) was studied in a two-phase system of benzyl alcohol-1N HCl solution. It was found that the distribution coefficient of Ac-D-PhG did not practically depend on its co...

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Bibliographic Details
Published inAntibiotiki Vol. 27; no. 1; p. 6
Main Authors Satarova, D E, Skvortsova, E E, Nys, P S, Korchagin, V B, Savitskaia, E M
Format Journal Article
LanguageRussian
Published Russia (Federation) 01.01.1982
Subjects
Acylation
Amino Acids - analysis
Amino Acids - isolation & purification
Glycine - analogs & derivatives
Glycine - analysis
Glycine - isolation & purification
Hydrolysis
Mathematics
Polarography - methods
Stereoisomerism
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Summary:The equilibrium distribution of acetyl-D-phenylglycine prepared with stereospecific hydrolysis of acetyl-D,L-phenylglycine (Ac-D,L-PhG) was studied in a two-phase system of benzyl alcohol-1N HCl solution. It was found that the distribution coefficient of Ac-D-PhG did not practically depend on its concentration in the aqueous phase. This means that Ac-D-PhG does not form associates in the organic phase. The studies provided development of an extraction polarimetric method for quantitative determination of Ac-D-PhG. The method implies extraction of Ac-D-PhG with benzyl alcohol from a strong acid medium (1N HCl solution), polarimetric determination of the equilibrium concentration of Ac-D-PhG in the organic phase and subsequent calculation of the initial concentration of Ac-D-PhG in the aqueous phase with regard to its distribution coefficient.
ISSN:0003-5637