Diastereomers of nonionic oligonucleotide analogs. VI. Isolation of diastereomers of ethyl phosphotriesters of the octanucleotide d(GCCAAACA) by high performance affinity chromatography

Diastereomers of oligonucleotide ethyl phosphotriesters were separated by high-performance complementary (affinity) chromatography on a column with the immobilized complementary oligonucleotide. The elution buffer contained 0.18 M K2HPO4, pH 7.5, and 30% acetonitrile. The temperature of the separati...

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Published inBioorganicheskaia khimiia Vol. 18; no. 1; p. 92
Main Authors Vtorushina, I A, Gorbunov, Iu A, Zarytova, V F, Komarova, N I, Lebedev, A V, Lokhov, S G, Manaenko, A V, Siniakov, A N, Shishkina, I G
Format Journal Article
LanguageRussian
Published Russia (Federation) 01.01.1992
Subjects
Chromatography, Affinity
Chromatography, High Pressure Liquid
Oligonucleotides - chemistry
Organophosphorus Compounds - chemistry
Stereoisomerism
Temperature
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Summary:Diastereomers of oligonucleotide ethyl phosphotriesters were separated by high-performance complementary (affinity) chromatography on a column with the immobilized complementary oligonucleotide. The elution buffer contained 0.18 M K2HPO4, pH 7.5, and 30% acetonitrile. The temperature of the separation was a few degrees lower than Tm of corresponding oligonucleotide complexes. The diastereomers separated completely or partially were: d[GCC(Et)AAACA], d[GCCA(Et)AACA], d[GCAA(Et)ACA], d[GCC(Et)A(Et)AACA], d[GCC(Et)AA(Et)ACA], d[GCCA(Et)A(Et)ACA], d[GCC(Et)A(Et)A(Et)ACA].
ISSN:0132-3423