Detection of Toxoplasma gondii DNA in body fluids by polymerase chain reaction

The polymerase chain reaction (PCR) is used for in vitro amplification of specific DNA fragments. The PCR technique is based on the reiteration of a three-step process: denaturation DNA into single strands, annealing primers (specific synthetic oligonucleotides) to the DNA template and enzymatic ext...

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Bibliographic Details
Published inWiadomości parazytologiczne Vol. 41; no. 1; p. 13
Main Author Gołab, E
Format Journal Article
LanguagePolish
Published Poland 1995
Subjects
AIDS-Related Opportunistic Infections - complications
AIDS-Related Opportunistic Infections - diagnosis
Animals
Body Fluids - parasitology
DNA, Protozoan - analysis
Humans
Polymerase Chain Reaction - methods
Toxoplasma - genetics
Toxoplasma - isolation & purification
Toxoplasmosis - complications
Toxoplasmosis - diagnosis
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Summary:The polymerase chain reaction (PCR) is used for in vitro amplification of specific DNA fragments. The PCR technique is based on the reiteration of a three-step process: denaturation DNA into single strands, annealing primers (specific synthetic oligonucleotides) to the DNA template and enzymatic extension from the primers along the templates. The usefulness of this new technique to the detection of Toxoplasma gondii DNA has been described.
ISSN:0043-5163