A comparative evaluation of an immunoenzyme analytical method using a fluorogenic or chromogenic substrate in determining the Marburg virus antigen

A comparative evaluation of the developed variants of indirect solid-phase enzyme immunoassay using fluorogenic and chromogenic substrates for the determination of Marburg virus antigen was carried out. The resolving capacity of this method was 3.8 x 10(-9) g of protein for the former and 3.1 x 10(-...

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Bibliographic Details
Published inVoprosy virusologiĭ Vol. 38; no. 1; p. 17
Main Authors Kutuzov, V A, Mikhaĭlov, V V, Kruglova, S E, Kutuzov, V V, Kirillov, A P, Krasnianskiĭ, V P, Pshenichnov, V A, Lebedinskaia, E V
Format Journal Article
LanguageRussian
Published Russia (Federation) 01.01.1993
Subjects
Animals
Antigens, Viral - analysis
Ebolavirus - immunology
Evaluation Studies as Topic
Hymecromone - analogs & derivatives
Immunoenzyme Techniques
Indicators and Reagents
Marburgvirus - immunology
Nitrophenols
Organophosphorus Compounds
Rabbits
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Summary:A comparative evaluation of the developed variants of indirect solid-phase enzyme immunoassay using fluorogenic and chromogenic substrates for the determination of Marburg virus antigen was carried out. The resolving capacity of this method was 3.8 x 10(-9) g of protein for the former and 3.1 x 10(-8) g of protein for the latter substrate. Cross titrations demonstrated the lack of common antigens with Ebola virus.
ISSN:0507-4088