Activity of liver microsomal Ca2+-ATPase in relation to perturbation of membrane hydrophobic interactions induced by methoxybenzene derivatives and n-aliphatic alcohols

Changes in the activity of liver microsomal Ca2+-ATPase were studied in the presence of two series of lipophilic compounds: four flavouring substances derived from methoxybenzene and four n-aliphatic alcohols. With each compound the activity was stimulated at lower concentrations and inhibited at hi...

Full description

Saved in:
Bibliographic Details
Published inToxicology letters Vol. 44; no. 1-2; p. 31
Main Authors Fernandez, Y, Cambon-Gros, C, Deltour, P, Mitjavila, S
Format Journal Article
LanguageEnglish
Published Netherlands 01.11.1988
Subjects
Alcohols - pharmacology
Animals
Benzene Derivatives - pharmacology
Calcium-Transporting ATPases - metabolism
Electron Spin Resonance Spectroscopy
In Vitro Techniques
Intracellular Membranes - drug effects
Male
Microsomes, Liver - drug effects
Microsomes, Liver - enzymology
Rats
Rats, Inbred Strains
Online AccessGet more information

Cover

More Information
Summary:Changes in the activity of liver microsomal Ca2+-ATPase were studied in the presence of two series of lipophilic compounds: four flavouring substances derived from methoxybenzene and four n-aliphatic alcohols. With each compound the activity was stimulated at lower concentrations and inhibited at higher concentrations. The linear relationship between equiactive concentrations of the compounds and their partition coefficients showed that the enzyme activity was modulated by perturbation of membrane hydrophobic interactions. Measurements carried out by electron-spin resonance (ESR) showed evidence of a decrease in the membrane order induced by these compounds. However, results obtained with the methoxybenzene derivatives showed that the modification in ATPase activity cannot be directly related to the decrease in membrane order. This decrease did not only reflect perturbation of hydrophobic interactions.
ISSN:0378-4274
1879-3169