Synthesis, cloning, and expression in Escherichia coli cells of human alpha1-antitrypsin cDNA

cDNA coding for the full-length human alpha 1-antitrypsin (AAT) and its leader sequence has been cloned and sequenced. DNA sequences encoding the deletion variants and a full-length copy of AAT were cloned and expressed under trp-promoter control. It has been shown that guanine replacing right upstr...

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Bibliographic Details
Published inMolekulârnaâ genetika, mikrobiologiâ i virusologiâ no. 3; p. 36
Main Authors Tikunova, N V, Golovin, S Ia, Mikriukov, N N, Il'ichev, A A
Format Journal Article
LanguageRussian
Published Russia (Federation) 01.07.1996
Subjects
alpha 1-Antitrypsin - biosynthesis
alpha 1-Antitrypsin - genetics
Amino Acid Sequence
Base Sequence
Cloning, Molecular
DNA, Complementary
Escherichia coli - genetics
Escherichia coli - metabolism
Gene Expression
Humans
Molecular Sequence Data
Promoter Regions, Genetic
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Summary:cDNA coding for the full-length human alpha 1-antitrypsin (AAT) and its leader sequence has been cloned and sequenced. DNA sequences encoding the deletion variants and a full-length copy of AAT were cloned and expressed under trp-promoter control. It has been shown that guanine replacing right upstream and downstream the ATG of deletion variant increases the expression to ten-fold. The synthesis of deletion AAT in E. coli was evaluated immunologically and the level of the synthesis was shown to be 4-5% of total cellular protein.
ISSN:0208-0613