Determination of mutation frequency at loci of glycophorin A and T-cell receptors: informativeness for biological dosimetry of acute and prolonged irradiation

The frequencies of somatic mutations at loci of glycophorin A (GPA) and T-cell receptor (TCR) were determined in persons exposed professionally to ionizing radiation or a result of accidents at nuclear power plants and in control donors. Dependence of glycophorin A mutant (NO) cell frequency on dose...

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Published inRadiat͡s︡ionnai͡a︡ biologii͡a︡, radioėkologii͡a Vol. 38; no. 2; p. 171
Main Authors Saenko, A S, Zamulaeva, I A, Smirnova, S G, Orlova, N V, Selivanova, E I, Nugis, V Iu, Nadezhina, N M
Format Journal Article
LanguageRussian
Published Russia (Federation) 01.03.1998
Subjects
Adolescent
Adult
Aged
Data Interpretation, Statistical
Flow Cytometry
Glycophorin - genetics
Glycophorin - radiation effects
Humans
Linear Models
Middle Aged
Mutation
Occupations
Power Plants
Radiation Dosage
Radiation Injuries - diagnosis
Radiation Injuries - genetics
Radioactive Hazard Release
Radiometry
Receptors, Antigen, T-Cell - genetics
Receptors, Antigen, T-Cell - radiation effects
Software
Time Factors
Ukraine
Ukraine
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Summary:The frequencies of somatic mutations at loci of glycophorin A (GPA) and T-cell receptor (TCR) were determined in persons exposed professionally to ionizing radiation or a result of accidents at nuclear power plants and in control donors. Dependence of glycophorin A mutant (NO) cell frequency on doses of acute (up to 3.5 Gy) and prolonged (up to 15.0 Gy) irradiation was shown. The slope of linear regression corresponded to increase of NO-mutant frequency by 31.1 x 10(-6)/Gy (r = 0.87, p < 0.0001) for acute irradiation and by 6.3 x 10(-6)/Gy (r = 0.61, p < 0.0001) for prolonged one. The 5-fold decrease of the linear regression slope in the case of prolonged irradiation makes significantly worse permissive ability of the GPA test. Therefore its use for biological dosimetry of prolonged irradiation is not expedient in dose interval up to 1 Gy. The frequency of mutations in genes of T-cell receptor significantly correlated with dose of irradiation only in group of donors with recent radiation exposure (r = 0.75, p = 0.0002). Meanwhile, the TCR method is more sensitive and informative for biological dosimetry of recent radiation, than the GPA test.
ISSN:0869-8031