Patterns in the x-ray induction of mutations in resistance to 5-thioguanine in Chinese hamster cell cultures studied using cysteamine

X-ray induction of 5-thioguanine resistance mutations and the protective effect of cysteamine were studied in cultured cells of chinese hamster clone 431. Expression of mutations was studied for 8--10 days of post-irradiated growth, when cells surviving radiation at doses 200, 400, 600, 800 R were p...

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Bibliographic Details
Published inGenetika Vol. 17; no. 6; p. 1044
Main Authors Elisova, T V, Feoktistova, T P
Format Journal Article
LanguageRussian
Published Russia (Federation) 1981
Subjects
Animals
Cells, Cultured - radiation effects
Cricetinae
Cricetulus
Cysteamine - pharmacology
Dose-Response Relationship, Radiation
Drug Resistance - radiation effects
Mutation
Thioguanine - antagonists & inhibitors
Time Factors
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Summary:X-ray induction of 5-thioguanine resistance mutations and the protective effect of cysteamine were studied in cultured cells of chinese hamster clone 431. Expression of mutations was studied for 8--10 days of post-irradiated growth, when cells surviving radiation at doses 200, 400, 600, 800 R were passing from 3 to 12 generations. The maximum frequency of induced mutations was found after 3-5 generations. After reaching the maximum, the number of registered mutations decreased with the number of cell generations. Pre-irradiated treatment with 5mM cysteamine decreased induction of mutations 2--4 times when using doses 200--800 R. The frequency of mutations induced by mere radiation increased non-linearly with increasing doses, while in the presence of cysteamine the linear dose dependence was observed.
ISSN:0016-6758