The detection of viral antigens and ribosomal RNA in cells by using low-temperature media

A post-embedding technique for immunocytochemical analysis at the ultrastructural level was used to detect and localize HIV antigens on ultrathin sections of Lowicryl-embedded HIV-infected cells. A genomic probe containing ribosomal sequences and labeled with biotin was used to hybridize rRNA molecu...

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Bibliographic Details
Published inT͡S︡itologii͡a Vol. 32; no. 11; p. 1111
Main Authors Grigor'ev, V B, Kadoshnikov, Iu P, Escaig, F, Fournier, J G, Rudneva, I A, Karamov, E V, Kazennova, E V, Garaev, M M
Format Journal Article
LanguageRussian
Published Russia (Federation) 1990
Subjects
Acrylic Resins
Animals
DNA Probes
Gene Expression Regulation - genetics
HIV Antigens - analysis
HIV-1 - immunology
HIV-2 - immunology
Humans
Methods
Nucleic Acid Hybridization
RNA, Ribosomal - analysis
RNA, Ribosomal - genetics
Temperature
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Summary:A post-embedding technique for immunocytochemical analysis at the ultrastructural level was used to detect and localize HIV antigens on ultrathin sections of Lowicryl-embedded HIV-infected cells. A genomic probe containing ribosomal sequences and labeled with biotin was used to hybridize rRNA molecules in sections of animal cells embedded in Lowicryl. The method presently described offers the possibility to detect rapidly and precisely ribosomal gene expression and viral proteins at the ultrastructural level.
ISSN:0041-3771