Transformation of Bacillus thuringiensis by electroporation

Plasmids were transformed by electroporation into various strains of Bacillus thuringiensis with frequencies of up to 10(5) transformants/micrograms. pC 194 transformed all strains tested at a high frequency and cells could be stably transformed with pC194 and pUB110 simultaneously by electroporatio...

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Bibliographic Details
Published inFEMS microbiology letters Vol. 49; no. 2-3; p. 171
Main Authors Bone, E J, Ellar, D J
Format Journal Article
LanguageEnglish
Published England 01.04.1989
Subjects
Bacillus thuringiensis - genetics
Bacterial Proteins
Bacterial Toxins
Electricity
Endotoxins - genetics
Gene Expression Regulation
Genes, Bacterial
Hemolysin Proteins
Plasmids
Species Specificity
Transformation, Genetic
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Summary:Plasmids were transformed by electroporation into various strains of Bacillus thuringiensis with frequencies of up to 10(5) transformants/micrograms. pC 194 transformed all strains tested at a high frequency and cells could be stably transformed with pC194 and pUB110 simultaneously by electroporation with a frequency of 10(2) pC194+ pUB110 transformants/micrograms DNA. Low transformation frequencies observed with some plasmids, especially those grown initially in Escherichia coli, could be increased by passage through B. thuringiensis, B. thuringiensis var. israelensis and in acrystalliferous mutant of the same strain transformed at frequencies of 10(4)-10(5)/micrograms DNA with most of the plasmids tested. A cloned israelensis 27-kDa delta-endotoxin gene was introduced into the israelensis acrystalliferous mutant and a kurstaki acrystalliferous mutant by electroporation. Both transformants were shown to express the endotoxin gene and to be toxic to Aedes aegypti larvae.
ISSN:0378-1097