Inheritance of hybrid plasmid pAS-21 by cells of the purple phototropic nitrogen-fixing bacterium Rhodopseudomonas sphaeroides

Clones of purple nitrogenfixing prototrophic bacterium Rhodopseudomonas sphaeroides carrying an incertion into the chromosome of the hybrid pAS8-121 (RP4-ColE1 (repA::Tn7] plasmid were analysed. It is revealed that plasmid integration could be due to both Tn7 and other migrating elements (IS8 and po...

Full description

Saved in:
Bibliographic Details
Published inGenetika Vol. 20; no. 11; p. 1783
Main Authors Dubeĭkovskiĭ, A N, Kameneva, S V
Format Journal Article
LanguageRussian
Published Russia (Federation) 01.11.1984
Subjects
Chromosomes, Bacterial - ultrastructure
Cloning, Molecular
Conjugation, Genetic
DNA Transposable Elements
DNA, Bacterial - genetics
Escherichia coli - genetics
Hybridization, Genetic
Plasmids
Rhodobacter sphaeroides - genetics
Online AccessGet more information

Cover

More Information
Summary:Clones of purple nitrogenfixing prototrophic bacterium Rhodopseudomonas sphaeroides carrying an incertion into the chromosome of the hybrid pAS8-121 (RP4-ColE1 (repA::Tn7] plasmid were analysed. It is revealed that plasmid integration could be due to both Tn7 and other migrating elements (IS8 and possibly, to resident migrating elements of purple bacteria). The plasmid pAS 8-121 can be autonomously transferred from the cointegrate state into Escherichia coli K-12; the plasmid is not inherited autonomously in cells of the purple bacterium. In E. coli cells R' derivatives of the plasmid carrying R. sphaeroides chromosomal fragments may be formed. The R' plasmids with fragments of plasmid DNA substituted for chromosomal material of R. sphaeroides were selected in E. coli K-12 cells among deleted (Kms) derivatives of pAS8-121.
ISSN:0016-6758