Comparison of biological activity of high molecular weight and low molecular weight forms of immunoreactive prolactin from human serum in cultured lymphoma NB2 cells

In experiments with NB2 rat lymphoma cells culture sensitive to lactogenic hormones the mitogenic activity of high molecular weight (> OOK) immunoreactive prolactin, found in substantial quantities in serum of certain hyperprolactinemic women, as compared to the activity of serum low molecular we...

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Published inVoprosy meditsinskoi khimii Vol. 42; no. 3; p. 234
Main Authors Bulatov, A A, Osipova, T A, Makarovskaia, E E
Format Journal Article
LanguageRussian
Published Russia (Federation) 01.07.1996
Subjects
Animals
Antibodies, Monoclonal - pharmacology
Cell Division - drug effects
Female
Humans
Hyperprolactinemia - blood
Lymphoma
Molecular Weight
Prolactin - blood
Prolactin - isolation & purification
Prolactin - pharmacology
Rats
Tumor Cells, Cultured - drug effects
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Summary:In experiments with NB2 rat lymphoma cells culture sensitive to lactogenic hormones the mitogenic activity of high molecular weight (> OOK) immunoreactive prolactin, found in substantial quantities in serum of certain hyperprolactinemic women, as compared to the activity of serum low molecular weight (23K) form, was studied. It was established that the ratio of immunoreactive to biologically active prolactin content in serums in cases of low molecular weight form predominance is close to 1,0 whereas in case of predominant content of high molecular weight form it is substantially higher (1.5-2.3), apparently because of low biological activity of high molecular weight form. Direct comparison of mitogenic effects of equivalent quantities of serum immunoreactive prolactin forms with high and low molecular weight, separated by gel-filtration, confirmed low biological activity of high molecular weight form. Monoclonal antibodies to prolactin completely suppressed mitogenic activity of low molecular weight form and only partially--high molecular weight one. The data obtained indicate that high and low molecular weight forms of human serum immunoreactive prolactin differ in their biochemical and functional characteristics. Therefore their ratio in the circulating blood can substantially affect the clinical manifestations of hyperprolactinemia.
ISSN:0042-8809