Method of staining for lysosomal cationic proteins in histological paraffin sections of biopsies

A method is suggested for a histochemical and immunofluorescent detection of lysosome cation proteins in the histologic paraffin sections of biopsies. The method is based on the staining of histologic sections obtained from the material fixed in glutaraldehyde buffered with an alcohol solution of a...

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Bibliographic Details
Published inArkhiv patologii Vol. 48; no. 7; p. 82
Main Authors Pigarevskiĭ, V E, Mazing, Iu A, Seliverstova, V G
Format Journal Article
LanguageRussian
Published Russia (Federation) 1986
Subjects
Biopsy
Duodenum - metabolism
Duodenum - pathology
Fluorescent Antibody Technique
Gastric Mucosa - metabolism
Gastric Mucosa - pathology
Histocytochemistry
Humans
Intestinal Mucosa - metabolism
Intestinal Mucosa - pathology
Lysosomes - metabolism
Paraffin
Proteins - metabolism
Staining and Labeling - methods
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Summary:A method is suggested for a histochemical and immunofluorescent detection of lysosome cation proteins in the histologic paraffin sections of biopsies. The method is based on the staining of histologic sections obtained from the material fixed in glutaraldehyde buffered with an alcohol solution of a fast green (pH 8.1-8.2) and on the detection myeloperoxidase and lactoferrine by means immune serum by Coons's method. 0.25% solution of azur A (B, C) is used for staining the cell nuclei and viable bacteria.
ISSN:0004-1955