Acidification kinetics in macrophage phagosomes based on flow cytometric data

Using flow cytofluorimetry, acidification in macrophages was studied during phagocytosis of yeast cells Candida albicans conjugated with FITC. Two phases of acidification were found: one corresponding presumably to yeast cell absorption on the macrophage surface, and the other one being due to cell...

Full description

Saved in:
Bibliographic Details
Published inT͡S︡itologii͡a Vol. 25; no. 6; p. 707
Main Authors Filatov, A V, Khramtsov, A V, Senchenkov, E P, Zemskov, V M
Format Journal Article
LanguageRussian
Published Russia (Federation) 01.06.1983
Subjects
Animals
Ascitic Fluid - cytology
Candida albicans
Flow Cytometry
Hydrogen-Ion Concentration
Kinetics
Macrophages - metabolism
Mice
Mice, Inbred CBA
Organoids - metabolism
Phagocytosis
Online AccessGet more information

Cover

More Information
Summary:Using flow cytofluorimetry, acidification in macrophages was studied during phagocytosis of yeast cells Candida albicans conjugated with FITC. Two phases of acidification were found: one corresponding presumably to yeast cell absorption on the macrophage surface, and the other one being due to cell incorporation into phagolysosomes. The phases are characterized by pH values of 6.3 +/- 0.5 and 4.5 +/- 0.5, respectively. Addition of 10 mM NH4Cl cancelled the observed acidification, whereas the presence of cytochalasin B (2 . 10(-5) M) prevented the very process of acidification.
ISSN:0041-3771