The purification of beta-galactosidase by flow electrophoresis

A plant has been constructed for the free-flow electrophoresis. It permits carrying out electrophoretic separation both in a free flow and with the use of neutral grained fillers. Beta-galactosidase has been purified from the culture liquid of the Escherichia coli cell phage lysate. Its content in t...

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Bibliographic Details
Published inMikrobiologicheskii zhurnal Vol. 52; no. 2; p. 94
Main Authors Andrienko, V I, Gorlov, Iu I, Gavrish, T G
Format Journal Article
LanguageRussian
Published Ukraine 01.03.1990
Subjects
beta-Galactosidase - analysis
beta-Galactosidase - isolation & purification
Densitometry
Electrophoresis - instrumentation
Electrophoresis, Polyacrylamide Gel
Equipment Design
Escherichia coli - enzymology
Galactosidases - isolation & purification
Humans
Hydrogen-Ion Concentration
Infant, Newborn
Temperature
Ukraine
Ukraine
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Summary:A plant has been constructed for the free-flow electrophoresis. It permits carrying out electrophoretic separation both in a free flow and with the use of neutral grained fillers. Beta-galactosidase has been purified from the culture liquid of the Escherichia coli cell phage lysate. Its content in the purified material amounted to 81.2
ISSN:0201-8462