Quantitative determination of cattle angiogenin

Polyclonal antibodies to a milk antigen were obtained by a standard immunization procedure. The possibility was demonstrated to use the peroxidase-antibody conjugate in a competitive immunoenzymatic test. The minimal amount of the antigen determined by this method is 10 ng/ml.

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Bibliographic Details
Published inBiokhimiia (Moscow, Russia) Vol. 60; no. 8; p. 1344
Main Authors Rogov, I A, Shalygina, A M, Tikhomirova, N A, Rustam'ian, Iu L, Skorobogat'ko, O V, Mikhaĭlov, A M, Stepanova, E V, Komolova, G S
Format Journal Article
LanguageRussian
Published Russia (Federation) 01.08.1995
Subjects
Angiogenesis Inducing Agents - metabolism
Animals
Cattle
Immunoenzyme Techniques
Milk - metabolism
Proteins - metabolism
Ribonuclease, Pancreatic
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Summary:Polyclonal antibodies to a milk antigen were obtained by a standard immunization procedure. The possibility was demonstrated to use the peroxidase-antibody conjugate in a competitive immunoenzymatic test. The minimal amount of the antigen determined by this method is 10 ng/ml.
ISSN:0320-9725