Low incidence of mutations in codon 12 of the c-K-ras gene in bladder cancer

During vesical carcinogenesis a variety of genetic alterations such as oncogene mutation or loss of suppressor genes have been detected. Codon 12 mutation of the c-K-ras gene has been seen with a high frequency in several human neoplasias but its participation in the development of vesical cancers h...

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Bibliographic Details
Published inActas urologicas españolas Vol. 19; no. 8; p. 599
Main Authors Ejarque, M J, Villanueva, A, Chechile, G, Regalado, R, Errando, C, Algaba, F, Capella, G, Lluis, F, Villavicencio, H, Vicente, J
Format Journal Article
LanguageSpanish
Published Spain 01.09.1995
Subjects
Aged
Aged, 80 and over
Base Sequence
Carcinoma, Transitional Cell - genetics
Codon
DNA Mutational Analysis
Female
Genes, ras - genetics
Humans
Incidence
Male
Middle Aged
Molecular Sequence Data
Mutation
Urinary Bladder Neoplasms - genetics
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Summary:During vesical carcinogenesis a variety of genetic alterations such as oncogene mutation or loss of suppressor genes have been detected. Codon 12 mutation of the c-K-ras gene has been seen with a high frequency in several human neoplasias but its participation in the development of vesical cancers has not been fully dilucidated. Using the DNA restriction fragments polymorphism (RFLP) technique enhanced by a polymerase chain reaction (PCR) a study has been made of codon 12 mutation at the c-K-ras gene in 55 patient with vesical cancer undergoing surgery between 1991 and 1992. The tumoral stage was superficial (Ta-Tl) in 24 cases, infiltrant (T2-T4) in 28 cases and unknown in 3 cases. Two patients (3.6%) showed codon 12 mutation at the c-K-ras gene. One case was a fast evolving infiltrant tumour (T2-T3) which caused death of the patient after 4 months while the other case was a surface tumour (G2Ta) which relapsed early, the pathological anatomy revealing a stage T2-T3 squamous carcinoma. Our results suggest that codon 12 mutation at the c-K-ras gene is not a meaningful genetic change in the genesis of vesical cancer. Its emergence, however, appears to be related to a more aggressive tumoural behaviour.
ISSN:0210-4806