Culture of Aspergillus parasiticus in apple juice. II. The effect of butylhydroxyanisole (BHA) and butylhydroxytoluol (BHT) on fungal growth and aflatoxin biosynthesis

Aspergillus parasiticus (NRRL 2999) was incubated in apple juice (from syrup), quiescently at 25 degrees C for up to 15 days in the presence of butylated hydroxyanisole (BHA) or butylated hydroxytoluene (BHT) at concentrations of 100, 200, 300, or 400 mg/l. Mycelial dry weight, pH and concentration...

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Bibliographic Details
Published inZeitschrift für Lebensmittel-Untersuchung und -Forschung Vol. 185; no. 3; p. 223
Main Authors Velikonja, J, Pupovac-Velikonja, A, Dürrigl, A
Format Journal Article
LanguageGerman
Published Germany 01.09.1987
Subjects
Aflatoxin B1
Aflatoxins - biosynthesis
Aspergillus - drug effects
Aspergillus - growth & development
Aspergillus - metabolism
Beverages - analysis
Butylated Hydroxyanisole - pharmacology
Butylated Hydroxytoluene - pharmacology
Culture Media
Food Microbiology
Fruit - analysis
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Summary:Aspergillus parasiticus (NRRL 2999) was incubated in apple juice (from syrup), quiescently at 25 degrees C for up to 15 days in the presence of butylated hydroxyanisole (BHA) or butylated hydroxytoluene (BHT) at concentrations of 100, 200, 300, or 400 mg/l. Mycelial dry weight, pH and concentration of aflatoxin B1 and G1 were measured every 3 days with the initial pH of 2.5 remaining unchanged in all samples. BHA suppressed fungal growth and toxin accumulation during the observed incubation period. However, a concentration-dependent growth delay and earlier peaks of toxin accumulation suggested environmental adaptation of the mould. BHT (from 200 mg/l onwards), led to growth inhibition by about 25% (solubility limit of BHT lies between 200 and 300 mg/l), and at a concentration of 200 mg/l, it led to a reduction of toxin accumulation by approximately 45%. At 100 mg/l, however, BHT stimulated aflatoxin production (1.90 times more G1 and 6.65 times more B1 than in the controls). At all tested concentrations of BHA or BHT, as well as in the controls, the accumulation of aflatoxin G1, without exception, surpassed that of B1.
ISSN:0044-3026