Assessment of mitochondrial function in the surviving cytoplasts of cultured cells of pig embryonic kidney by using ethylrhodamine

• Published in: T͡S︡itologii͡a Vol. 28; no. 11; p. 1246
• Main Authors: Neverova, M E, Zorov, D B
• Format: Journal Article
• Language: Russian
• Published: Russia (Federation) 01.11.1986
• Subjects: Animals; Cell Line; Cell Nucleus - physiology; Cell Survival; Cells, Cultured; Kidney - ultrastructure; Mitochondria - physiology; Rhodamines; Staining and Labeling - methods; Swine; Time Factors; Xanthenes
• ISSN: 0041-3771

A study was made of the functional state of chondriome in cytoplasts previously cultivated for a long time under vital staining with fluorescent dye ethylrhodamine (10 micrograms/ml) which is known to accumulate preferentially in mitochondria. The energization was estimated by the intensity of mitochondrial fluorescence. It was realized that 30 minutes after enucleation cytoplasts retained the same fluorescence as did the untreated cells, and that the mitochondrial distribution within the cell was very similar. Such a high intensity of fluorescence seen within one day after enucleation gives a strong evidence on the high degree of independence on the cell nucleus of organelles that provide the energy to metabolic processes. In the course of survival in the cultivation medium for 1-4 days the intensity of fluorescence is shown to fall, especially on the second day after enucleation. All these changes coincide with the changes in cytoplast shape: originally well spread bodies transform into squeezed, ball-shaped or strongly deformed ones. The adhesive ability is going down, and in result only single units of cytoplast can hardly be found on the cover slips by the 4th day after enucleation. These changes give evidence on the enhanced degeneration of cultured cytoplasts.